Protein Expression and Purification Facility (PEP)

Protein expression & purification facility provides services for expression and purification of recombinant proteins. We offer large-scale production of proteins inEscherichia coli and Pichiapastorisexpression system. The scale of protein production is from milligrams to one gram per litre. Additionally, the facility is able to purify large amount of tagged and non-tagged recombinant proteins by affinity chromatography, ion exchange chromatography and size-exclusion chromatography. 


Key Instrumentation

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INFORS Bioreactors (2 to 30 litres)
ÄKTA Protein Purification Systems (GE)
SARTORIUS Crossflow Filtration Unit
IKA T25 Ultra Turrax Homogenizer
BRANSON Ultrasonic Cell Disruptor



Recombinant Protein Expression

Protein production using Escherichia coli and Pichia pastoris expression system.
Our services cater especially to structural biology, functional assays, antigen production and protein-protein interaction.


Protein Purification

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The protein purification facility is equipped with FPLCs and a wide assortment of both high and low grade chromatography columns, covering the vast majority of protein purification needs (affinity capture/ ion exchange/ gel filtration).
The process will include application to column, elution, buffer exchange (if necessary), desalting, sterile filtration and OD measurement. In addition, users may supply their own columns for specialized projects.   
 

Service Process 

 Confidentiality and Security

Strict confidentiality and security is guaranteed with every project we complete. MGI will not claim ownership of all related intellectual property. Data will be destroyed  within 30 days after completion, unless requested otherwise by customer.  

We strive to deliver first class products together with professional, fast and competent customer support whenever you need it. Feel free to contact us via email or phone to have our team to assist you.

For further information please contact :

  • Mohd Razif Mamat  (Scientist) – Expression
  • Farahayu Khairuddin (Scientist) - Purification


Malaysia Genome Institute (MGI)
National Institute of Biotechnology Malaysia (NIBM) 
Jalan Bangi,
43000 Kajang,
Selangor Darul Ehsan, MALAYSIA

 
You may also email us at:

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   +6 03 8926 7446 ext. 3204      Fax : +6 03 8926 7972